微通道下的硬脂酸酯水解动力学

微通道下的硬脂酸酯水解动力学(任务书,开题报告,论文10000字)
摘要
微反应器的传热能力和传质能力高，在微反应器中物料可以被瞬间均匀混合并且能够高效传热，许多很难在常规条件下进行的反应都可以在微反应器中发生[1]。因此微反应器被广泛应用在化学、化工、生物等诸多领域的研究和生产过程中，并且表现出很好的优势，酶促反应就是其中的一类[1]。这类反应在酶和微反应器的作用下，反应效果好，能够实现安全高产。但很少有人研究微反应器中酶促反应的动力学参数。所以为了探究动力学参数的变化，我们使用B型脂肪酶催化剂对硬脂酸酯进行两相催化水解反应，在微通道和常规条件下比较其水解的动力学参数Km、Vmax，最后通过计算发现在常规和微反中的动力学参数Km很相似，而Vmax的值微反要高于常规。当Vmax提高，反应到达平衡的时间就会降低。所以微反应器能够在不改变酶的Km参数的情况下缩短反应时间，降低反应物消耗。
关键词：微反应器  水解反应  动力学参数  脂肪酶435  两相反应
 
Hydrolysis Kinetics of Stearate in Microchannels
Abstract
The microreactor has the excellent capability of heat transfer and mass transfer , enabling instantaneous and uniform mixing the materials of reaction and efficient heat transfer. Many of the reactions that can not be achieved on bench scale can be achieved in microreactors. So micro-reactor has been widely used in chemistry, chemical, biological, materials and many other areas on research and production process, and has showna good development of prospect.The enzymatic reaction is one of them.         Theeffect of reactioncan be very goodin the help of enzyme and microreactor,safety and high yield. Even so,few people studied the nature of enzymatic reactions in microreversion. Therefore,in order toexplore the change of kinetic parameters, we use the B-type lipase catalyst to catalyze the hydrolysis of the stearate. And we compare the hydrolysis of kinetic parameters Km,Vmaxbetween the micro-channel and conventional conditions. we found that the kinetic parameters Km between the bench scale and micro-inverse are very similarby detecting,the value of Vmax is slightly higher than that of conventional. When Vmax is increased,the time to reach the equilibrium will be reduced. The microreactor is able to shorten the reaction time and reduce the reactant consumption without changing the Km parameter of the enzyme . [来源：http://think58.com]
Key words:microreactor; hydrolysis; kinetic parameter; lipase; Two-phase reaction 
目  录
摘要    I
Abstract    II
第一章  绪论    1
1.1  前言    1
1.2 微反应器研究    1
1.2.1 微反应器定义    1
1.2.2 微反应器优点    2
1.2.3 微反应器的选择性    2
1.2.4  Labtrix® Start——微通道反应器    3
1.3 酶微反应器    5
第二章  实验部分    7
2.1 模板反应    7
2.2 在常规中进行反应    8
2.2.1 常规中的酶反应动力学方程    8
2.2.2试剂和仪器    9
2.2.3 实验步骤    10
2.2.4 实验结果与讨论    10
2.2.5 小结    12
2.3 在微反中进行反应    12
2.3.1 微反中的动力学方程    12