斑点叉尾鮰肠套叠致病菌的初步研究

斑点叉尾鮰肠套叠致病菌的初步研究(8200字)
摘要：从发生斑点叉尾鮰肠套叠病鱼的肠、肝、肾内分离到3株致病性的菌株(C0801，C0802，C0803），经人工感染致病性试验证实C0801，C0802为斑点叉尾鮰肠套叠的病原菌。经鉴定C0801菌株为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia）。药敏实验结果表明：C0801对复方新诺明（SMZ-TMP），氟苯尼考(FLO），洛美沙星(LFM），强力霉素(DC），阿齐霉素(AZI）高度敏感；对四环素（TET）、诺氟沙星（NOR）中度敏感；对庆大霉素（GEN）、丁胺卡那（A）和妥布霉素（TOB）低度敏感；对青霉素、链霉素、麦迪霉素等具有耐药性。关键词: 斑点叉尾鮰；肠套叠；病原菌；药敏试验
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Channel Catfish Pathogens preliminary study of intussusception
Abstract：In the present study, 3 pathogenic strains (C0801, C0802, C0803) were isolated from the intestine, liver, kidney of Ictalurus punctatus that was infected by the communicable disease.It was verified that C0801 was pathogenic strain causing intestinal intussusception for Ictalurus punctatus. The results from sensitivity test showed that Stenotrophomonas maltophilia was highly sensitive to florfenicol, sulfamethoxazole, sulfisoxazol, azithromycin, lomefloxacin, sulfamethoxazole compound, doxycycline. It was moderately sensitive to sarafloxacin hydrochloric and tetracycline norfloxacin and low sensitive to gentamicin and amikacin. Meanwhile, the strain showed drug resistance to penicillin, streptomycin and midecamycin.

Key words: Ictalurus Punctatus; Intussusception; Pathogenic bacteria; Drug Sensitive Test

目    录
摘要    4
关键词    4
1. 材料与方法    5
1.1 试验材料    5
1.1.1 患病斑点叉尾鮰    5
1.1.2 健康斑点叉尾鮰    5
1.1.3  培养基、试剂、药敏纸片    6
1.2 试验方法    6
1.2.1 病原菌的分离与纯化    6
1.2.2 人工感染试验    6
1.2.3 菌种鉴定    7
1.2.4 药物敏感性试验    7
1.2.5 几种药物的最小抑菌、杀菌浓度    7
1.2.6 生产防治性试验    8
2. 结果与分析    8
2.1 病鱼的症状    8
2.2 人工感染试验    9
2.2.1 滤液人工感染试验（病毒学试验）    9
2.2.2 人工注射感染试验    9
2.2.3 人工浸泡感染试验    9
2.2.4 回归感染试验    10
2.3 分离菌的鉴定与生理生化特性    10
2.4 药物敏感性试验    12
2.5 最抑菌、杀菌浓度    13
3. 讨论    14
参考文献：    15
致    谢    17